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  • Increase of protein extraction yield from rapeseed meal through a pretreatment with phytase.

Increase of protein extraction yield from rapeseed meal through a pretreatment with phytase.

Journal of the science of food and agriculture (2016-10-16)
Ivo M Rodrigues, M Graça Vs Carvalho, Jorge Ms Rocha
ABSTRACT

Rapeseed meal is a good source of high-quality vegetal protein but contains antinutritional compounds that limit its use for human and animal feed. The aim of this study was to develop a methodology to enhance alkaline protein extraction of rapeseed meal and to produce protein-rich products with low levels of phytic acid. Different phytase dosages and operating conditions were used for rapeseed meal pretreatment followed by alkaline extraction at different temperatures, time, pH and solid/liquid ratios (S/L). The highest protein extraction yield attained was 72.1%, for 2 h at 55 °C, with a phytase dosage of 0.8 U g Phytase pretreatment enhanced alkaline extraction yield of proteins from rapeseed meal. This procedure allowed also the production of rapeseed protein concentrates with very low levels of phytic acid, ∼1 g kg

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phytase from wheat, ≥0.01 unit/mg solid