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Molecular and cellular impact of Psoriasin (S100A7) on the healing of human wounds.

Experimental and therapeutic medicine (2017-06-02)
Aravindan Rangaraj, Lin Ye, Andrew James Sanders, Patricia Elaine Price, Keith Gordon Harding, Wen Guo Jiang
ABSTRACT

Psoriasin, which is also known as S100A7, is a member of the S100 protein family, a group of calcium-responsive signalling proteins. Psoriasin expression remains high in patients with psoriasis, whereas it is downregulated in patients with invasive breast carcinoma. This observation suggests that this protein may be a notable marker of keratinocyte function and differentiation during wound healing. The aim of the present study was to determine the cellular impact of Psoriasin in keratinocytes, which are the primary cell type associated with wound healing. Psoriasin expression in wound tissues was examined using reverse transcription-quantitative polymerase chain reaction and immunochemical staining. Knockdown of Psoriasin in HaCaT cells was performed using anti-Psoriasin ribozyme transgenes and the effect on growth, adhesion and migration of keratinocytes was subsequently determined using

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
REDTaq® ReadyMix PCR Reaction Mix, Complete PCR reagent with standard Taq DNA Polymerase and inert dye
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Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution