• Home
  • Search Results
  • Clear cell renal cell carcinoma: a comparative study of histological and chromosomal characteristics between primary tumors and their corresponding metastases.

Clear cell renal cell carcinoma: a comparative study of histological and chromosomal characteristics between primary tumors and their corresponding metastases.

Virchows Archiv : an international journal of pathology (2017-05-11)
Julien Dagher, Solène-Florence Kammerer-Jacquet, Frédéric Dugay, Marion Beaumont, Alexandra Lespagnol, Laurence Cornevin, Grégory Verhoest, Karim Bensalah, Nathalie Rioux-Leclercq, Marc-Antoine Belaud-Rotureau
ABSTRACT

Clear cell renal cell carcinoma (ccRCC) has a poor prognosis with a 50% risk of metastases. Little is known about the phenotypic and molecular profiles of metastases regarding their corresponding primary tumors. This study aimed to screen phenotypic and genotypic differences between metastases and their corresponding primary tumors. We selected four cases with available frozen material. The histological, immunohistochemical (VEGFA, CD31, SMA, Ki67, p53, PAR-3), FISH (VHL gene), next-generation sequencing (VHL and c-MET genes), multiplex ligation-dependent probe amplification, and array-(comparative genomic hybridization) CGH analyses were realized. Metastases were nodal, hepatic (synchronous), adrenal, and pulmonary (metachronous). High-grade tumor cells were significantly more frequent in metastases (p = 0.019). Metastases and high-grade zones of primary tumors shared similar characteristics compared to low-grade zones: a lower microscopic vascular density (43.5 vs 382.5 vessels/mm

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Solketal methacrylate, 50 wt. % in dichloromethane, contains ~280 ppm 4-tert-butylcatechol as inhibitor
Sigma-Aldrich
Anti-VEGFA antibody produced in goat, affinity isolated antibody, buffered aqueous solution