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Phenolic metabolism and molecular mass distribution of polysaccharides in cellulose-deficient maize cells.

Journal of integrative plant biology (2017-05-06)
María de Castro, Romina Martínez-Rubio, José L Acebes, Antonio Encina, Stephen C Fry, Penélope García-Angulo
ABSTRACT

As a consequence of the habituation to low levels of dichlobenil (DCB), cultured maize cells presented an altered hemicellulose cell fate with a lower proportion of strongly wall-bound hemicelluloses and an increase in soluble extracellular polymers released into the culture medium. The aim of this study was to investigate the relative molecular mass distributions of polysaccharides as well as phenolic metabolism in cells habituated to low levels of DCB (1.5 μM). Generally, cell wall bound hemicelluloses and sloughed polymers from habituated cells were more homogeneously sized and had a lower weight-average relative molecular mass. In addition, polysaccharides underwent massive cross-linking after being secreted into the cell wall, but this cross-linking was less pronounced in habituated cells than in non-habituated ones. However, when relativized, ferulic acid and p-coumaric acid contents were higher in this habituated cell line. Feasibly, cells habituated to low levels of DCB synthesized molecules with a lower weight-average relative molecular mass, although cross-linked, as a part of their strategy to compensate for the lack of cellulose.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Xylobiose, ≥90% (HPLC)