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Periodic harvesting of microalgae from calcium alginate hydrogels for sustained high-density production.

Biotechnology and bioengineering (2017-05-04)
Scott C Pierobon, Jason Riordon, Brian Nguyen, Matthew D Ooms, David Sinton
ABSTRACT

High-density biomass production is currently only realized in biofilm-based photobioreactors. Harvest yields of whole biofilms are self-limited by daughter-upon-parent cell growth that hinders light and leads to respiratory biomass losses. In this work, we demonstrate a sustainable multi-harvest approach for prolonged generation of high-density biomass. Calcium-alginate hydrogel cultures loaded with Synechococcus elongatus PCC 7942 achieved production densities comparable to that of biofilms (10

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Alginic acid sodium salt from brown algae, BioReagent, suitable for plant cell culture, low viscosity, powder