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Frequently Asked Questions (FAQs)
- What ECM protein is the best for my cells?
While laminin and fibronectin are widely used ECM proteins, the one that your cell needs should be determined by test experiments. Stem cells express varied integrins on the surface during different stages of differentiation. The choice of ECM protein would depend on the stage of differentiation you are interested in studying.
- What is the purity of laminin products?
We do not determine purity of laminin solutions. An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots to ensure consistent quality.
- What is the best solvent to dilute laminin?
The solvents that are compatible to dilute laminins are provided in Table 1.
- Can laminin be reused?
Reuse of laminin is not recommended as there will be changes in the sterility and concentration and structure of laminin. Diluted, unused laminin can be kept at 2 - 8 °C for up to 1 week. Laminin-coated plates can be stored at 2 - 8 °C for up to 4 weeks provided they are sealed well to prevent contamination and/or drying up of laminin. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.
- What is the optimum concentration of laminin for coating?
The coating concentration of our laminins are suggested in Table 1. However, optimizing the concentrations that work for your cells is recommended.
- What is the best ECM protein to study neural differentiation?
Laminin or Fibronectin along with poly-D-lysine or poly-L-lysine support in vitro neural differentiation well. Refer to the protocol for detailed steps to induce neural differentiation.
For additional questions contact our technical services here.