Figure 1.Cryopreservation of Cell Lines
The protocol below describes the use of cell freezing methods involving an electric -80 °C freezer for the cryopreservation of cell lines. ECACC routinely use a programmable rate-controlled freezer. This is the most reliable and reproducible way to freeze cells but as the cost of such equipment is beyond the majority of research laboratories the methods below are described in detail. If large numbers of cell cultures are regularly being frozen then a programmable rate-controlled freezer is recommended.
Figure 2.DMSO Bottles
Figure 3.Mr. Frosty Containers
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