The following statements pertain specifically to Avanti’s lipid products for research and should not necessarily be applied to cGMP lipids intended for pharmaceutical or diagnostic applications.
Phospholipids supplied as an organic solution should be stored in a glass container layered with argon or nitrogen at -20 °C ± 4 °C. Storage of organic solutions below -30 °C is not recommended unless the solution is packaged in a sealed glass ampoule. The closure for the vial should be lined with teflon. Organic solutions should never be stored in polymer or plastic containers (polystyrene, polyethylene, polypropylene, etc.) as this will leach impurities out of the container. Lipids in water can be stored in plastic.
Always use glass, stainless steel, or teflon to transfer lipids stored as organic solutions.
Phospholipids supplied as a powder should be handled as described below.
Lipids composed of fatty acids that are completely saturated (e.g., dimyristoyl or dipalmitoyl phosphatidylcholine) are stable as powders. These lipids should be stored in a glass container with a teflon closure at ≤ -16 °C. When transferring a portion of the material out of the container, remove the container from the freezer and allow the material to reach room temperature before opening the bottle.
Lipids composed of fatty acids containing one or more double bonds (e.g., dioleoyl phosphatidylcholine or egg phosphatidylcholine) are not stable as powders. These lipids are extremely hygroscopic as powders and will quickly absorb moisture and become gummy upon opening the container. This could result in hydrolysis or oxidation of the material. These lipids should be dissolved in a suitable organic solvent, transferred to a glass container with a teflon closure, and stored at -20 °C ± 4 °C. Storage of organic solutions below -30 °C is not recommended unless the solution is packaged in a sealed glass ampoule.
Phospholipids should not be stored for long periods of time as aqueous suspensions. Storage of lipids in an excess of water results in hydrolysis of the sample.