Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.
Assign specific amino acid methyl groups separately or simultaneously using kits that connect region- or stereo- specific labeled 13CH3 groups to the 15N/13C backbone nuclei via a linear 13C spin system.
Figure 1. Optimized labeling of Ile, Leu, and Val for assignment. Protein was produced in U-[2H,13C,15N] M9 media supplemented with TLAM-Iγ2LVproR-U-[13C] kit.1,2
Achieve region and stereospecific labeling of Ile, Leu, Val, and Ala with 13CHD2 isotopomers. Acquire
high resolution solution and solid-state 1H, 13C spectra to investigate precise dynamics.
Investigate protein assemblies with molecular weight up to 1 MDa.
Use a SLAM kit to achieve high incorporation of 13CH3 isotopomer on a single type of methyl group. Use in perdeuterated minimal media without detectable scrambling.
Reduce spectral overlap using combinations of single labeling (SLAM) kits and simultaneously label different types of methyl probes that are well separated in Methyl-TROSY spectra.
Figure 2. Kits for Large Protein Labeling
Extract precise and long-range nOe distant restraints between methyl probes in perdeuterated proteins. Or, filter intra- and inter- molecular nOes and get more precise structural information to refine your homo-oligomeric protein structure.
User-friendly solutions for the simultaneous isotope labeling of combinations of Ala, Ile, Leu, Met, Thr, and Val methyl groups
NMR-Bio kits include region-specific and/or stereo-specific labeling of Ile, Leu, and Val residues. Kits are provided with precise protocols extensively tested in-vivo to ensure optimal incorporation in targeted methyl groups without detectable scrambling in other positions.
The kits are specifically deuterated and supplied frozen and ready to use. All kits are calibrated for addition into deuterated M9 culture medium prior to induction. These kits incorporate 13CH3 or 13CHD2 isotopomers into selected protein methyl groups. Protocols are provided with each kit, and each kit is designed for 1L of culture.
Figure 3. Detection of long-range nOes between methyl probes distant by up to 10 Å in large proteins and complexes.1,3