Anti-FAK Antibody, clone 4.47

clone 4.47, Upstate®, from mouse


Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


4.47, monoclonal

species reactivity

mouse, rat, human




immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... PTK2(5747)

General description

Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.


Recognizes p125FAK; does not cross react with Pyk-2.


GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK


Anti-FAK Antibody, clone 4.47 is an antibody against FAK for use in IC, IH, IP & WB.
Research Sub Category
Cytoskeletal Signaling
Research Category


Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates

Target description

125 kDa


Replaces: 04-591

Physical form

Protein G Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Positive Control Included: 3T3 Cell Lysate (12-305). Other controls: A31 cell lysate, PC-12, Hep-G2, SW 620 and Jurkat RIPA cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Targeted inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines.
Yoon, H; Choi, YL; Song, JY; Do, I; Kang, SY; Ko, YH; Song, S; Kim, BG
Testing null
Y Shen et al.
Molecular biology of the cell, 10(8), 2507-2518 (1999-08-06)
The focal adhesion kinase (FAK) is discretely localized to focal adhesions via its C-terminal focal adhesion-targeting (FAT) sequence. FAK is regulated by integrin-dependent cell adhesion and can regulate tyrosine phosphorylation of downstream substrates, like paxillin. By the use of a...
Sanjeev K Baniwal et al.
PloS one, 8(6), e62361-e62361 (2013-06-12)
Prolactin-induced Protein (PIP), an aspartyl protease unessential for normal mammalian cell function, is required for the proliferation and invasion of some breast cancer (BCa) cell types. Because PIP expression is particularly high in the Luminal A BCa subtype, we investigated...
Nicoletta I Petridou et al.
PloS one, 7(8), e42577-e42577 (2012-08-11)
The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic development and targeted disruption of the...
B van de Water et al.
The Journal of biological chemistry, 274(19), 13328-13337 (1999-05-01)
The relationship between focal adhesion protein (FAK) activity and loss of cell-matrix contact during apoptosis is not entirely clear nor has the role of FAK in chemically induced apoptosis been studied. We investigated the status of FAK phosphorylation and cleavage...

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