Documents

AB5603

Sigma-Aldrich

Anti-Sox2 Antibody

Chemicon®, from rabbit

Synonym(s):
Transcription factor SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

rabbit

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

application(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

Transcription factor SOX-2 (UniProt: P48431; also known as SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2) is encoded by the SOX2 gene (Gene ID: 6657) in human. SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. It may function as a switch in neuronal development. It allows neural cells in undifferentiated state by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Sumoylation of SOX-2 is shown to inhibit binding on DNA and negatively regulate the FGF4 transactivation. Defects in SOX2 gene are linked to microphthalmia that is characterized by eye malformation ranging from small eye size of a single eye to complete bilateral absence of ocular tissue.

Specificity

This antibody recognizes Sox2 C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.

Application

Anti-SOX2 Antibody, Cat. No. AB5603, is a highly specific rabbit polyclonal antibody SOX2 and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson′s disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).

Quality

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SOX2 in 10 µg of mouse embryonic stem cell lysate.

Target description

~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates

Linkage

Replaces: AB5770

Analysis Note

Control
Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
Immunocytochemistry: Human embryonic stem cells (H9 line)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation.
Wong YH, Lu AC, Wang YC, Cheng HC, Chang C, Chen PH, Yu JY, Fann MJ
The Journal of Neuroscience null
Cell lineage mapping of taste bud cells and keratinocytes in the mouse tongue and soft palate.
Tadashi Okubo, Cheryl Clark, Brigid L M Hogan, Tadashi Okubo, Cheryl Clark, Brigid L M Hogan
Stem Cells null
Cerebellar stem cells act as medulloblastoma-initiating cells in a mouse model and a neural stem cell signature characterizes a subset of human medulloblastomas.
Sutter, R, et al.
Oncogene, 29, 1845-1856 (2010)
A directional Wnt/beta-catenin-Sox2-proneural pathway regulates the transition from proliferation to differentiation in the Xenopus retina.
Agathocleous, Michalis, et al.
Development, 136, 3289-3299 (2009)
Tgfbeta signal inhibition cooperates in the induction of iPSCs and replaces Sox2 and cMyc.
Nimet Maherali,Konrad Hochedlinger
Current Biology null
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