Anti-Ephrin-B2 Antibody

clone 10H1.1, from mouse

Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


10H1.1, monoclonal

species reactivity

human, mouse, rat


immunohistochemistry: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... EFNB2(1948)

General description

Ephrin-B2 is also called EPH-related receptor tyrosine kinase ligand 5 (LERK-5) and HTK ligand (HTK-L). Ephrin-B2 is a cell surface transmembrane ligand for the Eph tyrosine kinase family of receptors. Ephrin-B2 binds Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling, which is critical for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-B2 is expressed in lung and kidney and together with EPHB4 is involved in heart morphogenesis and angiogenesis.


KLH-conjugated linear peptide corresponding to human Ephrin-B2.


Anti-Ephrin-B2, clone 10H1.1, Cat. No. MABC127, is a mouse monoclonal antibody that detcets Ephrin-B2 and has been tested for use in immunohitochemistry and Western Blotting.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ephrin-B2 in rat and human lung tissue lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ephrin-B2 in IHC.


Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ephrin-B2 in 10 µg of mouse lung tissue lysate.

Target description

~65 kDa observed. The calculated molecular weight of this protein is ~37 kDa; however, this protein has been observed at 65-70 kDa (Attwood, B. K., et al. (2011). Nature. 473(7347):372–375.; Lin, K. T., et al. (2008). J Biol Chem. 283(43):28969-28979.).

Physical form

Purified by Ion-Exchange Chromatography
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Mouse lung tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Maha Coucha et al.
Vascular pharmacology, 131, 106761-106761 (2020-06-26)
Diabetes-induced retinal vascular cell death aggravates diabetic retinopathy (DR) to the proliferative stage and blindness. Pericytes play a crucial role in retinal capillaries survival, stability, and angiogenesis. Ephrin-B2 is a tyrosine kinase that regulates pericytes/endothelial cells communication during angiogenesis; yet...
Ling-Dan Dong et al.
Oncology letters, 13(4), 2109-2114 (2017-04-30)
Endometrial adenocarcinoma (EC) is one of the most frequently diagnosed types of endometrial cancer and is typically a consequence of continuous estrogen receptor stimulation. Erythropoietin-producing hepatocyte receptor B4 (EphB4) and its ligand ephrin-B2 have been reported to be overexpressed in...
Nadja Kemmerling et al.
Glia, 65(7), 1103-1118 (2017-04-04)
The Eph-ephrin system plays pivotal roles in cell adhesion and migration. The receptor-like functions of the ephrin ligands allow the regulation of intracellular processes via reverse signaling. γ-Secretase mediated processing of ephrin-B has previously been linked to activation of Src...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.