Ephrin-B2 is also called EPH-related receptor tyrosine kinase ligand 5 (LERK-5) and HTK ligand (HTK-L). Ephrin-B2 is a cell surface transmembrane ligand for the Eph tyrosine kinase family of receptors. Ephrin-B2 binds Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling, which is critical for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-B2 is expressed in lung and kidney and together with EPHB4 is involved in heart morphogenesis and angiogenesis.
KLH-conjugated linear peptide corresponding to human Ephrin-B2.
Anti-Ephrin-B2, clone 10H1.1, Cat. No. MABC127, is a mouse monoclonal antibody that detcets Ephrin-B2 and has been tested for use in immunohitochemistry and Western Blotting.
Research Sub Category
Apoptosis - Additional
Apoptosis & Cancer
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ephrin-B2 in rat and human lung tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ephrin-B2 in IHC.
Evaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ephrin-B2 in 10 µg of mouse lung tissue lysate.
~65 kDa observed. The calculated molecular weight of this protein is ~37 kDa; however, this protein has been observed at 65-70 kDa (Attwood, B. K., et al. (2011). Nature. 473(7347):372–375.; Lin, K. T., et al. (2008). J Biol Chem. 283(43):28969-28979.).
Purified by Ion-Exchange Chromatography
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Mouse lung tissue lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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