63064

Sigma-Aldrich

Magnesium chloride hexahydrate

BioUltra, ≥99.0% (KT)

Linear Formula:
MgCl2 · 6H2O
CAS Number:
Molecular Weight:
203.30
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

product line

BioUltra

Quality Level

assay

≥99.0% (KT)

impurities

insoluble matter, passes filter test
≤0.0002% total nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤2000 mg/kg
Li: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤3000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.030
λ: 280 nm Amax: 0.025

storage temp.

2-8°C

SMILES string

O.O.O.O.O.O.Cl[Mg]Cl

InChI

1S/2ClH.Mg.6H2O/h2*1H;;6*1H2/q;;+2;;;;;;/p-2

InChI key

DHRRIBDTHFBPNG-UHFFFAOYSA-L

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Application

  • Magnesium chloride hexahydrate has been used in wash buffer for the purification of protein from Saccharomyces cerevisiae.
  • It has been used for the preparation of extracellular (Ringer) solution for juxtacellular recording of single neurons in freely moving rats.
  • It has been used as a supplement to study effect of desiccation on bacterial viability.

Other Notes

Reconstitution of membrane processes; Strongly enhances the vinblastine-induced tubulin self-association; Component of Dulbecco′s phosphate buffered saline for tissue culture; Component of culture media for Pseudomonas. In Salmonella Enrichment Broth acc. to Rappaport, inhibits normal intestinal flora.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Physical properties of purified calf brain tubulin.
G C Na et al.
Methods in enzymology, 85 Pt B, 393-408 (1982-01-01)
Effect of air drying on bacterial viability: A multiparameter viability assessment.
Nocker A, et al.
Journal of Microbiological Methods, 90, 86-86 (2012)
G D Eytan et al.
The Journal of biological chemistry, 251(21), 6831-6837 (1976-11-10)
Cytochrome oxidase, QH2-cytochrome c reductase, and the oligomycin-sensitive adenosine triphosphatase were incorporated into liposomes by a new procedure which yielded unidirectional orientation of the proteins. Cytochrome oxidase was reconstituted in the mitochrondrial orientation and the adenosine triphosphatase in the submitochondrial...
Juxtacellular recording and morphological identification of single neurons in freely moving rats.
Tang Q, et al.
Nature Protocols, 9, 2369-2369 (2014)
In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease.
Chauhan S, et al.
Bio-protocol null

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