49290

Sigma-Aldrich

β-Glucosidase from almonds

lyophilized, powder, ≥4 U/mg

Synonym(s):
β-D-Glucoside glucohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

powder

Quality Level

quality

lyophilized

specific activity

≥4 U/mg

mol wt

Mr ~135000

storage temp.

2-8°C

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Application

β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.

Biochem/physiol Actions

βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol glucose per minute at pH 5.0 and 37 °C (salicin as substrate)

Other Notes

Role of β-glucosidase in cellulose hydrolysis: Review; Enzymic hydrolysis of cellulose for fuel ethanol production by simultaneous saccharification and fermentation; Stereospecific attachment of carbohydrates to amino acid derivatives; Enzyme-catalyzed synthesis of alkyl β-D-glucosides in organic media

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
G P Philippidis et al.
Biotechnology and bioengineering, 41(9), 846-853 (1993-04-15)
The biochemical conversion of cellulosic biomass to ethanol, a promising alternative fuel, can be carried out efficiently and economically using the simultaneous saccharification and fermentation (SSF) process. The SSF integrates the enzymatic hydrolysis of cellulose to glucose, catalyzed by the...
G. Vic, and Thomas, D.
Tetrahedron Letters, 33, 4567-4567 (1992)
Turner, N.J., and Webberley, M.C.
Journal of the Chemical Society. Chemical Communications, 1349-1349 (1991)
Beta-Glucosidase: its role in cellulase synthesis and hydrolysis of cellulose.
J G Shewale
The International journal of biochemistry, 14(6), 435-443 (1982-01-01)
Laura Nyström et al.
Journal of agricultural and food chemistry, 55(22), 9059-9065 (2007-10-03)
Rye and wheat bran are excellent natural sources of plant sterols in the diet. Their content, however, may vary according to processing. Thermal (roasting and heating in a microwave oven), mechanical (milling and cryogenic grinding), and enzymatic treatments (hydrolysis with...

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