CS1020

Sigma-Aldrich

Glutathione Assay Kit, Fluorimetric

sufficient for 200 multiwell tests

EC Number:
NACRES:
NA.84

Quality Level

usage

sufficient for 200 multiwell tests

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GSTA4(2941)

Application

The Glutathione Assay Kit, Fluorimetric includes all the reagents required for fast and easy measurement of reduced glutathione in cell or tissue extracts or directly in growing cells. Staurosporine is supplied with the kit to enable induction of apoptosis in cells. Since the amount of reduced glutathione in staurosporine-induced cells is significantly lower than in non-induced cells, this can serve as a control system.
Glutathione Assay Kit, Fluorimetric has been used in intracellular thiol assays and in the measurement of hepatic glutathione in rats.

Biochem/physiol Actions

Glutathione (GSH) acts as a cofactor for several antioxidant enzymes. It helps in the regeneration of vitamins C and E. It regulates cellular proliferation and apoptosis. GSH is important for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene is associated with Alzheimer′s, Parkinson′s and Huntington′s diseases.
Reduced glutathione (GSH), a tripeptide (γ-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues. Glutathione is present inside cells primarily in the reduced form (90-95% of the total glutathione). The remainder is present in the oxidized form (glutathione disulfide, GSSG). Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell and its ability to resist toxic challenge. High levels of GSH in the cell may indicate pathological changes.

Analysis Note

The kit assay utilizes a thiol probe (monochlorobimane), which can freely pass through the plasma membrane. The free, unbound probe shows very little fluorescence, but when bound to reduced glutathione in a reaction that is catalyzed by glutathione S-transferase (GST), it forms a strongly fluorescent adduct.

Kit Components Only

Product No.
Description

  • Assay Buffer 50 mL

  • Substrate Solution 1 mL

  • Lysis Buffer 10x 1.5 mL

  • Glutathione S-Transferase 1 mL

  • Staurosporine Ready Made 100 μL

  • Glutathione, reduced 300 mg

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Preparation of ZnO/Ag nanocomposite and coating on polymers for anti-infection biomaterial application
Sadeghi B.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 118, 787-792 (2014)
Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats
Park SJ, et al.
Journal of ginseng research, 37(1), 37-37 (2013)
Glutathione: Physiological and clinical relevance
Pizzorno JE and Katzinger JJ
Journal of restorative medicine, 1(1), 24-37 (2012)
Rajesh Parsanathan et al.
Archives of biochemistry and biophysics, 672, 108054-108054 (2019-07-28)
Hydrogen sulfide (H2S) is an endogenous novel gasotransmitter which is implicated in the pathophysiology of the metabolic syndrome. Core clock genes (CCG) and its controlled genes disruption is implicated in the progression of metabolic syndrome. We examined whether H2S has...
Glucose-6-Phosphate Dehydrogenase Deficiency Activates Endothelial Cell and Leukocyte Adhesion Mediated via the TGF?/NADPH Oxidases/ROS Signaling Pathway.
Parsanathan, et al.
International Journal of Molecular Sciences, 21 (2021)

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