M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

cation traces

heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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General description

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It was used to prepare buffer to lyse cells for immunoprecipitation and to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

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Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
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Protocols
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