SRP6549

Sigma-Aldrich

Alkaline Phosphatase from calf intestine

≥98% (SDS-PAGE)

Synonym(s):
ALP-1, ALPPL2, PLAP, ALP, PALP, ALPP, PLAP-1, ALPPL
EC Number:

biological source

bovine (calf) intestine

assay

≥98% (SDS-PAGE)

form

freeze-dried protein glass

packaging

pkg of 1 mg
pkg of 10 μg
pkg of 50 μg

impurities

3000-6,000 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

bovine (calf) ... ALPL(280994)

General description

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.

Application

Alkaline phosphatase from calf intestine has been used as a standard in alkaline phosphatase assay.

Biochem/physiol Actions

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.

Physical form

Lyophilized without any additives.

Reconstitution

Reconstitute in distilled water or dilute buffer.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion.
Pal S
Lab on a chip, 8, 1332-1341 (2008)
Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Cell host & microbe, 2, 371-382 (2007)
Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity.
Addison WN
The Journal of Biological Chemistry, 282, 15872-15883 (2007)
Affinity-chromatography purification of alkaline phosphatase from calf intestine.
Brenna O
The Biochemical Journal, 151, 291-296 (1975)
Intestinal alkaline phosphatase. Physical properties and quaternary structure.
M Fosset et al.
Biochemistry, 13(9), 1783-1788 (1974-04-23)

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