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Genome-lamina interactions are established de novo in the early mouse embryo.

Nature (2019-05-24)
Máté Borsos, Sara M Perricone, Tamás Schauer, Julien Pontabry, Kim L de Luca, Sandra S de Vries, Elias R Ruiz-Morales, Maria-Elena Torres-Padilla, Jop Kind

In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic interactions with the nuclear lamina (a filamentous meshwork that lines the inner nuclear membrane) in mouse pre-implantation embryos. We reveal that nuclear organization is not inherited from the maternal germline but is instead established de novo shortly after fertilization. The two parental genomes establish lamina-associated domains (LADs)4 with different features that converge after the 8-cell stage. We find that the mechanism of LAD establishment is unrelated to DNA replication. Instead, we show that paternal LAD formation in zygotes is prevented by ectopic expression of Kdm5b, which suggests that LAD establishment may be dependent on remodelling of H3K4 methylation. Our data suggest a step-wise assembly model whereby early LAD formation precedes consolidation of topologically associating domains.

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2-Mercaptoethanol, ≥99.0%
Fetal Bovine Serum, non-USA origin, sterile-filtered, suitable for cell culture
Puromycin dihydrochloride from Streptomyces alboniger, powder, BioReagent, suitable for cell culture
IGEPAL® CA-630, for molecular biology
TWEEN® 20, viscous liquid, suitable for cell culture
Magnesium acetate solution, BioUltra, for molecular biology, ~1 M in H2O
Indole-3-acetic acid sodium salt, BioReagent, suitable for plant cell culture, ≥98%
Potassium acetate solution, BioUltra, for molecular biology, 5 M in H2O
Trizma® acetate, ≥99.0% (titration)
5-Carboxy-tetramethylrhodamine N-succinimidyl ester, BioReagent, suitable for fluorescence

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