• Home
  • Search Results
  • EphA2-to-YAP pathway drives gastric cancer growth and therapy resistance.

EphA2-to-YAP pathway drives gastric cancer growth and therapy resistance.

International journal of cancer (2019-08-04)
Changhao Huang, Weijie Yuan, Chen Lai, Shangwei Zhong, Chen Yang, Ran Wang, Linfeng Mao, Zihua Chen, Zhikang Chen
ABSTRACT

Yes-associated protein (YAP) is a transcriptional coactivator that promotes cell proliferation, stem cell maintenance and tissue homeostasis. The YAP activity is primarily regulated through an inhibitory phosphorylation by the serine/threonine kinases of Hippo pathway. Here, we show that receptor tyrosine kinase (RTK) erythropoietin-producing hepatocellular receptor A2 (EphA2) interacts with and phosphorylates YAP protein, leading to stabilization, nuclear translocation and activation of YAP in gastric cancer (GC) cells. EphA2 induces chemotherapy-resistance by increasing YAP stability and nuclear YAP protein. Knockdown of YAP blocks EphA2-induced tumor growth in GC xenograft mouse models. Importantly, the coactivation of EphA2 and YAP is manifested in clinical human GC, and is related to GC recurrence. Thus, our results establish a novel EphA2-to-YAP pathway that drives GC growth, progression and therapy-resistance, targeting this pathway would be an efficient way for the treatment of GC, particularly chemotherapy-resistant GC.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal, purified from hybridoma cell culture, clone PYP-76
Sigma-Aldrich
MISSION® esiRNA, targeting human EPHA2

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.