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Optimization of β-glucosidase production by a strain of Stereum hirsutum and its application in enzymatic saccharification.

Journal of microbiology and biotechnology (2013-03-07)
Priyadharshini Ramachandran, Ngoc-Phuong-Thao Nguyen, Joon-Ho Choi, Yun Chan Kang, Marimuthu Jeya, Jung-Kul Lee

A high beta-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from S. hirsutum showed a higher level of activity to cellobiose (V(max) = 172 U/mg, and k(cat) = 281/s). Under the optimum conditions (600 rpm, 30°C, and pH 6.0), the maximum BGL activity of 10.4 U/ml with the overall productivity of 74.5 U/l/h was observed. BGL production was scaled up from a laboratory scale (7-L fermenter) to a pilot scale (70-L fermenter). When S. hirsutum was cultured in fed-batch culture with rice straw as the carbon source in a 70-L fermenter, a comparable productivity of 78.6 U/l/h was obtained. Furthermore, S. hirsutum showed high levels of activity of other lignocellulases (cellobiohydrolase, endoglucanase, xylanase, and laccase) that are involved in the saccharification of biomasses. Application of S. hirsutum lignocellulases in the hydrolysis of Pinus densiflora and Catalpa ovata showed saccharification yields of 49.7% and 43.0%, respectively, which were higher than the yield obtained using commercial enzymes.

Product Number
Product Description

Peptone from animal tissue, from meat, BioReagent, suitable for cell culture, suitable for plant cell culture
Peptone from casein and other animal proteins, suitable for microbiology
Peptone from milk solids, Refined hydrolysate
Peptone Primatone® RL, suitable for microbiology
Proteose Peptone, Enzymatic hydrolysate
Peptone from animal tissue, from meat
Peptone from animal tissue, BioReagent, Type I, suitable for plant cell culture, from meat
Peptone Primatone® RLT, suitable for microbiology

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