• Home
  • Search Results
  • Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion.

Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion.

Nature structural & molecular biology (2014-01-21)
Zahra Timsah, Zamal Ahmed, Chi-Chuan Lin, Fernando A Melo, Loren J Stagg, Paul G Leonard, Prince Jeyabal, Jonathan Berrout, Roger G O'Neil, Mikhail Bogdanov, John E Ladbury
ABSTRACT

FGFR2-expressing human cancer cells with low concentrations of the adaptor protein Grb2 show high prevalence for metastatic outcome. In nonstimulated cells, the SH3 domain (and not the SH2 domains) of Plcγ1 directly competes for a binding site at the very C terminus of FGFR2 with the C-terminal SH3 domain of Grb2. Reduction of Grb2 concentration permits Plcγ1 access to the receptor. Recruitment of Plcγ1 in this way is sufficient to upregulate phospholipase activity. This results in elevated phosphatidylinositol 4,5-bisphosphate turnover and intracellular calcium levels, thus leading to increased cell motility and promotion of cell-invasive behavior in the absence of extracellular receptor stimulation. Therefore, metastatic outcome can be dictated by the constitutive competition between Grb2 and Plcγ1 for the phosphorylation-independent binding site on FGFR2.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phospholipase C from Clostridium perfringens (C. welchii), Type I, lyophilized powder, 10-50 units/mg protein
Sigma-Aldrich
Phospholipase C from Bacillus cereus, ≥200 units/mg protein
Sigma-Aldrich
Phospholipase C from Clostridium perfringens (C. welchii), Type XIV, lyophilized powder, ≥150 units/mg protein

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.