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Probing RNA structure and metal-binding sites using terbium(III) footprinting.

Current protocols in nucleic acid chemistry (2008-04-23)
Dinari A Harris, Nils G Walter
ABSTRACT

The function of an RNA molecule is determined by its overall secondary and tertiary structure. The tertiary structure is facilitated and stabilized by the interaction with metal ions. The current chapter offers a detailed protocol on the use of the lanthanide metal ion terbium(III) as a powerful probe of RNA structure and metal-binding properties. When incubating RNA with low (micromolar) concentrations of terbium(III), specific backbone scission by partially deprotonated aqueous terbium(III) complexes can be used to detect high-affinity metal-binding sites, while incubation with high (millimolar) terbium(III) concentrations cleaves the RNA backbone preferentially at structurally accessible regions, providing a footprint of the RNA secondary and tertiary structure.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Terbium, chips, 99.9% trace rare earth metals basis
Sigma-Aldrich
Terbium, foil, size 25 mm × 25 mm × 1 mm, 99.9% trace rare earth metals basis
Terbium, foil, 25x25mm, thickness 0.1mm, as rolled, 99%
Terbium, foil, 50x50mm, thickness 0.1mm, as rolled, 99%
Terbium, foil, not light tested, 25x25mm, thickness 0.025mm, as rolled, 99%
Terbium, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99%
Terbium, powder, 1g, max. particle size 500 micron, 99.9%
Terbium, powder, 2g, max. particle size 500 micron, 99.9%
Terbium, powder, 5g, max. particle size 500 micron, 99.9%
Terbium, rod, 100mm, diameter 6.35mm, cast, 99%
Terbium, rod, 50mm, diameter 6.35mm, cast, 99%
Terbium, wire reel, 100mm, diameter 1.0mm, hard, 99.9%
Terbium, wire reel, 50mm, diameter 1.0mm, hard, 99.9%

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