• Home
  • Search Results
  • Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2.

Yes-associated protein regulates endothelial cell contact-mediated expression of angiopoietin-2.

Nature communications (2015-05-13)
Hyun-Jung Choi, Haiying Zhang, Hongryeol Park, Kyu-Sung Choi, Heon-Woo Lee, Vijayendra Agrawal, Young-Myeong Kim, Young-Guen Kwon
ABSTRACT

Angiogenesis is regulated by the dynamic interaction between endothelial cells (ECs). Hippo-Yes-associated protein (YAP) signalling has emerged as a key pathway that controls organ size and tissue growth by mediating cell contact inhibition. However, the role of YAP in EC has not been defined yet. Here, we show expression of YAP in the developing front of mouse retinal vessels. YAP subcellular localization, phosphorylation and activity are regulated by VE-cadherin-mediated-EC contacts. This VE-cadherin-dependent YAP phosphorylation requires phosphoinositide 3-kinase-Akt activation. We further identify angiopoietin-2 (ANG-2) as a potential transcriptional target of YAP in regulating angiogenic activity of EC in vitro and in vivo. Overexpression of YAP-active form in EC enhances angiogenic sprouting, and this effect is blocked by ANG-2 depletion or soluble Tie-2 treatment. These findings implicate YAP as a critical regulator in angiogenesis and provide new insights into the mechanism coordinating junctional stability and angiogenic activation of ECs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
Thrombin from human plasma, lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)
Sigma-Aldrich
MISSION® esiRNA, targeting human YAP1
Sigma-Aldrich
MISSION® esiRNA, targeting human YY1AP1
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Yap1

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.