98 atom % 15N

15N Labeled nitrogen
Linear Formula:
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

Quality Level

vapor density

1.34 (vs air)

isotopic purity

98 atom % 15N


99% (CP)

mass shift


SMILES string




InChI key


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Nitrogen-15N2 (15N Labeled nitrogen) can be used:
  • In nitrogen fixation experiments to monitor the resulting metabolites in an organism.
  • 15N2 pulse-chase experiments to understand the route of transport of fixed nitrogen from the nodule to the shoot in plants.
  • To quantify biological N2 production in agricultural soil.


250, 500 mL in Sure-Pac™
250 mL and 500 mL quantities are packaged in a 25 mL Sure/Pac cylinder with brass 1/4" male NPT valve. Nominal gas pressures at 21ºC are 135 psig for 250 mL and 275 psig for 500 mL.

Quantities of 1 L and 5 L are packaged in a 450 mL carbon steel lecture bottle with brass CGA 110/180 valve. Nominal gas pressures at 21ºC are 20 psig for I L and 155 psig for 5 L.

Cylinder pressures are slightly above atmospheric pressure. Care must be taken when extracting this product from the cylinder.
All gas volumes are quoted at Standard Temperature and Pressure (STP) and not at Normal Temperature and Pressure (NTP). One STP Liter is 7.7% more gas than one NTP Liter.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

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Legal Information

Sure/Pac is a trademark of Sigma-Aldrich Co. LLC


Gas cylinder





Press. Gas Compr. Gas


2A - Gases

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Producing and dispensing small quantities of 15N2 gas at atmospheric pressure
Diocares R, et al.
Analytical biochemistry, 352(2), 302-304 (2006)
Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using 15N as a tracer
Van Phi Hung N, et al.
Soil Science and Plant Nutrition, 59(6), 888-895 (2013)
In situ quantification of biological N2 production using naturally occurring 15N15N
Yeung LY, et al.
Environmental Science & Technology, 53(9), 5168-5175 (2019)
Min-Hyung Ryu et al.
Nature microbiology, 5(2), 314-330 (2019-12-18)
Legumes obtain nitrogen from air through rhizobia residing in root nodules. Some species of rhizobia can colonize cereals but do not fix nitrogen on them. Disabling native regulation can turn on nitrogenase expression, even in the presence of nitrogenous fertilizer...
Isabell Klawonn et al.
The ISME journal, 13(8), 1960-1974 (2019-03-27)
Ammonium is a central nutrient in aquatic systems. Yet, cell-specific ammonium assimilation among diverse functional plankton is poorly documented in field communities. Combining stable-isotope incubations (15N-ammonium, 15N2 and 13C-bicarbonate) with secondary-ion mass spectrometry, we quantified bulk ammonium dynamics, N2-fixation and...

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