This product is in 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
05-636
Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
clone JBW301, Upstate®, from mouse
Synonym(s):
H2AXS139P, Histone H2A.X (phospho S139)
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25 μG
1 730,00 kr
200 μG
8 540,00 kr
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
clone
JBW301, monoclonal
species reactivity
vertebrates
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
Related Categories
1 of 4
This Item | 16-202A | 05-636-I | 16-193 |
|---|---|---|---|
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified antibody |
| species reactivity vertebrates | species reactivity human | species reactivity human, rat, mouse | species reactivity human |
| clone JBW301, monoclonal | clone JBW301, monoclonal | clone JBW301, monoclonal | clone JBW301, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
| technique(s) ChIP: suitable, immunofluorescence: suitable, western blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable | technique(s) ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable |
General description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
17 kDa
Immunogen
peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
Application
Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Anti-phospho-Histone H2A.X (Ser139) Antibody clone JBW301, recognizes Histone H2A.X phosphorylated at Ser139.
Physical form
Format: Purified
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Protein G Purified
Preparation Note
Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Analysis Note
Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE205
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Your product quoted in https://doi.org/10.1016/j.mrgentox.2010.05.009 as 1:1000 dilution.if i use 05-636-25UG, how much µg/ml antibody shall I have to use for whole cell ELISA.
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For 05-636, we have not studied this internally for ELISA and therefore, do not have suggestions on using this for ELISA.
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