1.00286

L-Glutamine

EMPROVE^® ESSENTIAL DAB,USP

• Synonym(s): L-Glutamic acid-5-amide, Gln, (S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
• Linear Formula: H₂NCOCH₂CH₂CH(NH₂)CO₂H
• CAS Number: 56-85-9
• Molecular Weight: 146.14
• Beilstein: 1723797
• MDL number: MFCD00008044
• EC Index Number: 200-292-1

Properties

• Agency: DAB; USP
• Quality Level: 500
• product line: EMPROVE^® ESSENTIAL
• form: solid
• potency: 7500 mg/kg LD₅₀, oral (Rat)
• pH: 4.0-6.0 (20 °C, 25 g/L in H₂O)
• mp: 185 °C (dec.) (lit.)
• solubility: 100 g/L
• density: 1.47 g/cm³ at 20 °C
• bulk density: 640 kg/m³
• storage temp.: 2-30°C
• SMILES string: N[C@@H](CCC(N)=O)C(O)=O
• InChI: 1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
• InChI key: ZDXPYRJPNDTMRX-VKHMYHEASA-N

Description

General description

EMPROVE^® ESSENTIAL DAB,USP

Packaging

1 kg in PE bottle

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance)99.0 - 101.0 %Identity (IR-spectrum)passes testAppearancewhite to almost white, fine powderAppearance of solution (25 g/l; CO₂-free water)clear and colorlessSpec. rotation (α 20/D, 100 g/l, hydrochloric acid 2 mol/l, calc. on dried substance)+31.5 to +33.0Spec. rotation (α 20/D, 40 g/l, water, calc. on dried substance)+6.3 to +7.3pH (25 g/l; CO₂-free water)4.0 - 6.0Chloride (Cl)≤ 200 ppmSulfate (SO₄)≤ 200 ppmHeavy metals (as Pb)≤ 10 ppmCu (Copper)≤ 25 ppmFe (Iron)≤ 10 ppmFe (Iron) (ICP)≤ 10 ppmMn (Manganese)≤ 25 ppmNi (Nickel)≤ 2 ppmZn (Zinc)≤ 130 ppmNH₄ (Ammonium)≤ 0.1 %Ninhydrin - positive substances (TLC)≤ 0.5 %Residual solvents (ICH Q3C)excluded by manufacturing processSulfated ash (600 °C)≤ 0.1 %Loss on drying (105 °C; 3 h)≤ 0.3 %Bacterial endotoxins≤ 2.0 I.U./gElemental impurity specifications have been set considering ICH Q3D (Guideline for Elemental Impurities). Class 1-3 elements are not likely to be present above the ICH Q3D option 1 limit, unless specified and indicated (*).Corresponds to DAB, USP

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable