The temperature used for standard tissue processing historically has been room temperature. Temperature can affect the rate of fixation, and modern tissue processors may use elevated temperatures to reduce processing time without adverse effects on tissue morphology. Cold fixation is common in electron microscopy when using glutaraldehyde as a fixative.
1.00496
Formaldehyde solution 4%, buffered, pH 6.9
(approx. 10% Formalin solution) for histology
Synonym(s):
Formaline solution, Methanal solution, Methyl aldehyde solution
Sign In to View Organizational & Contract Pricing.
Select a Size
Pricing and availability is not currently available.
About This Item
NACRES:
NA.21
UNSPSC Code:
41116124
Bp:
100 °C/1013 hPa
Skip To
form
liquid
IVD
for in vitro diagnostic use
concentration
3.8-4.2% (acidimetric)
color
colorless
pH
6.7-7.1 ( in H2O)
bp
100 °C/1013 hPa
mp
-92 °C
density
1.017 g/cm3 at 20 °C
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| bp 100 °C/1013 hPa | bp 93-96 °C/1013 hPa | bp 93-96 °C/1013 hPa | bp 97 °C |
| density 1.017 g/cm3 at 20 °C | density 1.09 g/cm3 at 20 °C | density 1.09 g/cm3 at 20 °C, 1.09 g/mL at 25 °C (lit.) | density 1.09 g/mL at 25 °C (lit.) |
| mp -92 °C | mp <-15 °C | mp <-15 °C | mp -15 °C |
General description
Formaldehyde (HCOH) is one of the most familiar and effective fixatives. Formaldehyde fixation is suitable for nearly all types of histological tissue, as well as calcified deposits, bone, cartilage, nervous system specimens. The fixation of the histological specimen material of human or animal origin is commonly performed in a 4% or 10% Formaldehyde solution. Chemically, the 4 % Formaldehyde solution equals a 10 % Formalin solution. This Formaldehyde solution 4% is a ready-to-use working solution that is stabilized with phosphate buffer to protect tissue structures. The development of formic acid will be effective prevented. It is an IVD registered and CE certified product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
Storage Class
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
67-56-1
CAS No.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
Related Content
Learn about the criticality of biological tissue staining for research and clinical pathology using standard and special stains and dyes.
-
Is it okay to fix an entire tissue with 4% PFA at 37°C overnight using product 1004960700 without altering its morphology?
1 answer-
Helpful?
-
-
Do 4% formaldehyde or 10% formalin solutions contain traces of methanol?
1 answer-
Product 1004960700 and all other formaldehyde or formalin solutions contain methanol to prevent the formaldehyde from reforming as a polymer or escaping as a gas. If a methanol-free solution is required, paraformaldehyde powders or crystals can be purchased and prepared into methanol-free solutions, although these solutions are unstable and should be prepared fresh daily or kept for only short periods of time.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service