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MAB1567

Sigma-Aldrich

Anti-Myelin Associated Glycoprotein Antibody, clone 513

clone 513, Chemicon®, from mouse

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Synonym(s):
MAG
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

513, monoclonal

species reactivity

mouse, rat, frog, bovine, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAG(4099)

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SAB1402258SAB1401236FCMAB337F
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

513, monoclonal

clone

3C7, monoclonal

clone

polyclonal

clone

513, monoclonal

species reactivity

mouse, rat, frog, bovine, chicken, human

species reactivity

human

species reactivity

human

species reactivity

rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Milli-Mark®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

western blot: 1 μg/mL

technique(s)

flow cytometry: suitable

General description

Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.

Specificity

Detects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies.

Immunogen

L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.

Application

Detect Myelin Associated Glycoprotein using this Anti-Myelin Associated Glycoprotein Antibody, clone 513 validated for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins
Western Blot:
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}

Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by immunohistochemistry on rat hippocampus.

Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.

Target description

100 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat hippocampus tissue, rat brain lysate, PC12 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Gijsbert P van Nierop et al.
European journal of immunology, 46(2), 347-353 (2015-10-29)
MS pathology is potentially orchestrated by autoreactive T cells, but the antigens recognized remain unknown. A novel APC/T-cell platform was developed to determine intrathecal CD4(+) and CD8(+) T-cell responses to candidate MS-associated autoantigens (cMSAg) in clinically isolated syndrome (CIS, n
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Kristi Clark et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(29), 11788-11799 (2013-07-19)
Pelizaeus-Merzbacher disease (PMD) is a hypomyelinating leukodystrophy caused by mutations of the proteolipid protein 1 gene (PLP1), which is located on the X chromosome and encodes the most abundant protein of myelin in the central nervous sytem. Approximately 60% of
Katarina Vajn et al.
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Gangliosides - sialic acid-bearing glycolipids - are major cell surface determinants on neurons and axons. The same four closely related structures, GM1, GD1a, GD1b and GT1b, comprise the majority of total brain gangliosides in mammals and birds. Gangliosides regulate the
Marylens Hernandez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(3), 806-813 (2016-01-23)
Oligodendrocyte progenitors respond to biophysical or mechanical signals, and it has been reported that mechanostimulation modulates cell proliferation, migration, and differentiation. Here we report the effect of three mechanical stimuli on mouse oligodendrocyte progenitor differentiation and identify the molecular components

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