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MAB386

Sigma-Aldrich

Anti-Myelin Basic Protein Antibody, a.a. 82-87

culture supernatant, clone 12, Chemicon®

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Synonym(s):
MBP
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

12, monoclonal

species reactivity

human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)

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This Item
M3821M9434MAB382
antibody form

culture supernatant

antibody form

affinity isolated antibody

antibody form

tissue culture supernatant

antibody form

culture supernatant

clone

12, monoclonal

clone

polyclonal

clone

14, monoclonal

clone

1, monoclonal

species reactivity

human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all

species reactivity

rat, human, mouse

species reactivity

horse, chicken, human

species reactivity

bovine, rat, rabbit (weakly), human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable

technique(s)

flow cytometry: 1:200-1:500, immunohistochemistry: 1:100-1:200, western blot: 1:500-1:1,000

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, radioimmunoassay: suitable, western blot: suitable

General description

The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets.

Specificity

Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken.
Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV.

Immunogen

Bovine myelin basic protein
Epitope: a.a. 82-87

Application

Anti-Myelin Basic Protein Antibody, a.a. 82-87 is an antibody against Myelin Basic Protein for use in ELISA, IC, IH, RIA & WB.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.

Western Blotting:
Identifies an 18-20kDa band in westerns.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins

Quality

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.

Target description

18-20 kDa

Physical form

Liquid containing 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Arie C van Vliet et al.
Journal of neuropathology and experimental neurology, 74(9), 901-911 (2015-08-01)
Functional recovery does not occur in 10% of patients with neonatal brachial plexus palsy. In these patients, resection of a neuroma-in-continuity (NIC) and surgical nerve reconstruction are required. The formation of a NIC seems to prohibit functional recovery, but the
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