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MABE284

Sigma-Aldrich

Anti-MSH2 Antibody, clone FE11

clone FE11, from mouse

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Synonym(s):
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FE11, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MSH2(4436)

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NA26MABE324PLA0106
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

clone

FE11, monoclonal

clone

GB12, monoclonal

clone

1F6, monoclonal

clone

-

species reactivity

human

species reactivity

human

species reactivity

mouse

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

-

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): 2-10 μg/mg, western blot: 1:1,000-1:5,000

isotype

IgG1κ

isotype

IgG1

isotype

IgG1κ

isotype

-

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General description

MSH2 is a ubiquitously expressed nuclear protein that is involved in DNA repair processes. MSH2 is active as a heterodimer of MutS alpha and beta subunits that bind DNA at mismatched strands. The MutS alpha subunit binds to single base mismatches and dinucleotide insertion-deletion loops, whereas MutS beta detects longer insertion-deletion loops. Bound MutS subunits then form complexes with MutL alpha dimers, to coordinate downstream DNA-mismatch–repair processes. Abnormal expression of MSH2 has been linked to hereditary non-polyposis colorectal cancer type 1 and Muir-Torre syndrome.

Specificity

This antibody recognizes the C-terminus of MSH2.

Immunogen

Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human MSH2.

Application

Anti-MSH2 Antibody, clone FE11 is a Mouse Monoclonal Antibody for detection of MSH2 also known as DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2 & has been validated in WB & IHC.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SW-480 in human normal colonic tissue and in human adenocarcinoma tissue (Thibodeau, S. N., et al. (1996). 56(21):4836-4840.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MSH2 in 10 µg of SW-480 cell lysate.

Target description

~104 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
SW-480 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Sahra Bodo et al.
Oncotarget, 6(28), 24969-24977 (2015-09-04)
Mismatch-repair (MMR)-deficient cells show increased in vitro tolerance to thiopurines because they escape apoptosis resulting from MMR-dependent signaling of drug-induced DNA damage. Prolonged treatment with immunosuppressants including azathioprine (Aza), a thiopurine prodrug, has been suggested as a risk factor for
David Capper et al.
International journal of cancer, 133(7), 1624-1630 (2013-04-05)
The differentiation between hereditary and sporadic microsatellite-unstable (MSI-H) colorectal cancer is a crucial step in Lynch syndrome diagnostics. Within MSI-H colorectal cancers, the BRAF V600E mutation is strongly associated with sporadic origin. Here, we asked whether BRAF V600E-specific immunohistochemistry (clone
Kellie S Matthews et al.
Obstetrics and gynecology, 111(5), 1161-1166 (2008-05-02)
To estimate the frequency of mismatch repair deficiencies associated with hereditary nonpolyposis colorectal cancer, or Lynch syndrome, in women less than age 50 with endometrial cancer. Consecutive patients less than age 50 diagnosed with endometrial adenocarcinoma were identified. Available pathologic
Sang Jin Kim et al.
Annals of surgical treatment and research, 87(3), 123-130 (2014-09-24)
Sporadic colorectal cancers with high-frequency microsatellite instability (MSI-H) are related to hypermethylation of mismatch repair (MMR) genes and a higher frequency of BRAF mutations than Lynch syndrome. We estimated the feasibility of hereditary colorectal cancer based on hMLH1 methylation and
Y Parc et al.
Gut, 53(3), 371-375 (2004-02-13)
Microsatellite instability (MSI) has been identified as a factor with good prognosis and chemosensitivity in stage III C colon cancer. The purpose of this study was to evaluate the routine use of immunohistochemical analysis (immunohistochemical staining of MSH2 and MLH1)

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