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UFC5003

Millipore

Amicon Ultra-0.5 Centrifugal Filter Unit

Ultracel-3 regenerated cellulose membrane, 0.5 mL sample volume

eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

application(s)

protein extraction: suitable
protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm2

pore size

3 kDa NMWCO

test parameters

sample volume: 0.5 mL

shipped in

ambient

General description

AMICON ULTRA 0.5ML - 3KDa cutoff. Available in 4 pack sizes:
UFC500308 - 8 pack
UFC500324 - 24 pack
UFC500396 - 96 pack
UFC5003BK - 500 pack


Concentrates 500 µL down to 15 µL
Concentration factor 25 x to 30 x
High sample recovery (greater than 90% of dilute starting solution)
Fast processing time - 10 to 30 minutes
Reverse spin capability provides consistent recoveries

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates
Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
Desalting, buffer exchange and protein dialysis

Material Supplied:
The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.
Filter Type: Ultrafiltration

Application

Ultracel-3 regenerated cellulose membrane, 0.5 mL sample volume

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

Articles

Protein Labeling and Purification with Amicon® Pro Centrifugal Filters

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Amicon® Pro Purification System – Protein Purification

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Protocols

Small-scale Antibody Purification With Buffer Exchange and Concentration: Amicon® Pro System

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Detergent Removal with Amicon® Pro Centrifugal Filters

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

Related Content

Amicon® Ultra Centrifugal Filters

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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