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UFC5100

Millipore

Amicon Ultra-0.5 Centrifugal Filter Unit

Ultracel-100 regenerated cellulose membrane, 0.5 mL sample volume

Synonym(s):
Centrifugal concentrator, 100 kDa MWCO, 0.5 mL sample volume
eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

technique(s)

protein extraction: suitable
protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm2

pore size

100 kDa NMWCO

test parameters

sample volume: 0.5 mL

shipped in

ambient

General description

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

Linkage

Replaces: 42414

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

Articles

Protein Labeling and Purification with Amicon® Pro Centrifugal Filters

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Amicon® Pro Purification System – Protein Purification

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Protocols

Small-scale Antibody Purification With Buffer Exchange and Concentration: Amicon® Pro System

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Detergent Removal with Amicon® Pro Centrifugal Filters

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

Related Content

Amicon® Ultra Centrifugal Filters

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service