All Photos(1)

UFC8030

Millipore

Amicon® Ultra-4 Centrifugal Filter Unit

Ultracel-30 regenerated cellulose membrane, 4 mL sample volume

Synonym(s):
Centrifugal concentrator,30 kDa MWCO, 4 mL sample volume
eCl@ss:
36100101
NACRES:
NB.24

Quality Level

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

technique(s)

protein extraction: suitable
protein purification: suitable

L

124 mm (4.88 in.)

diam.

17.3 mm

filtration area

3 cm2

pore size

30 kDa NMWCO

test parameters

sample volume: 4 mL

shipped in

ambient

General description

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

  • Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipette sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

Articles

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

Protein Sample Ultrafiltration

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Protocols

Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters

Biomarkers play an essential role in the drug discovery and development process.

A Simple Strategy for Protein Enrichment Using Ultrafiltration

The human plasma proteome is a vast resource for biomarker discovery and investigation.

Related Content

Amicon® Ultra Centrifugal Filters

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service