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F9252

Sigma-Aldrich

Folin & Ciocalteu′s phenol reagent

suitable for determination of total protein by Lowry method, 1.9-2.1 N

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MDL number:
NACRES:
NA.47

form

liquid

Quality Level

concentration

1.9-2.1 N

color

clear yellow
faint yellow to very dark yellow, and Faint Green-Yellow to Very Dark Green-Yellow

pH

<0.5 (20 °C)

density

1.240 g/cm3 at 20 °C

suitability

suitable for determination of total protein by Lowry method

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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This Item
47641TP0200114529
Phenol Red ACS reagent

114529

Phenol Red

concentration

2 N

concentration

2 M (with respect to acid)

concentration

-

concentration

-

color

clear yellow

color

-

color

-

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

form

liquid

form

-

form

-

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

General description

Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
In the absence of copper, color intensity is determined primarily by the tyrosine and tryptophan content of the protein, and to a lesser extent by cysteine and histidine. Copper(II) has no effect on color formation by tyrosine, tryptophan, or histidine, but reduces color formation due to cysteine.
Many modifications of the original assay procedure have been published, including methods for enhancing the color development, for determining the content of insoluble proteins, and for automating the procedure. Compounds including many buffers, chelating agents, detergents, and cyclic organic compounds can interfere with the Lowry protein assay.
Folin & Ciocalteu′s phenol reagent can also be used as a spray reagent in chromatographic procedures.

Application

Folin & Ciocalteu′s phenol reagent has been used for the estimation of total phenolic content in samples.

Other Notes

Folin & Ciocalteu′s phenol reagent does not contain phenol. Rather, the reagent will react with phenols and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

Linkage

This product is also available as part of a kit.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes.
Sandhu D
Scientific Reports, 7, 42958-42958 (2017)
Analysis of phenolic compounds of interest in metabolism.
H G BRAY et al.
Methods of biochemical analysis, 1, 27-52 (1954-01-01)
Bioactive compounds and antioxidant activities during fruit ripening of watermelon cultivars.
Tlili I et al.
J. Food Compos. Anal., 24, 923-928 (2011)
E Larson et al.
Analytical biochemistry, 155(2), 243-248 (1986-06-01)
Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.
H J Fryer et al.
Analytical biochemistry, 153(2), 262-266 (1986-03-01)
The method of protein determination reported by Lowry et al. (1951, J. Biol. Chem. 193, 265-275) has been adapted for use with 96-well microtiter plates and an automatic microplate spectrophotometer. The spectrophotometer has been interfaced with a computer which plots

Protocols

To standardize a procedure for the determination of protein by modified Lowry.

Evaluate PCR inhibitors and enzyme selection using GenElute-E Single Spin Plant Extraction Kit, KOD-One PCR Master Mix, and competitors.

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Related Content

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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