Merck
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10771

Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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NACRES:
NA.75

sterility

sterile-filtered

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.077 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Histopaque-1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes. It may also be employed in the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.

Histopaque-1077 has been used for:
  • the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
  • purification of mononuclear cells through centrifugation
  • isolation of human lymphocytes
  • isolation of breast cancer circulating tumor cells (CTCs) from the whole blood
Histopaque-1077 and Histopaque-1119 has been used for neutrophil isolation from human blood through a double-density gradient. It has also been used for the isolation of pancreatic islets.It is also suitable for the purification of the rat mononuclear bone marrow stem cells for the investigation of spermatogenesis restoration.

Features and Benefits

Histopaque®-1077 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered ontoHistopaque-1077. During centrifugation, erythrocytes are aggregated by polysucroseand rapidly sediment. Granulocytes becomeslightly hypertonic, which increases their sedimentation rate, resulting in pelleting atthe bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain at the plasma/ Histopaque-1077interface.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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