Merck
All Photos(2)

Documents

11191

Sigma-Aldrich

Histopaque®-1119

sterile-filtered, density: 1.119 g/mL

All Photos(2)
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.119 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
10771PPB023MABF144A
Histopaque®-1119 sterile-filtered, density: 1.119 g/mL

Sigma-Aldrich

11191

Histopaque®-1119

Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Tris buffer Pre-set, pH 8.1, pHast Pack™, powder

Sigma-Aldrich

PPB023

Tris buffer

Anti-TER-119 (mouse), redFluor® 710, clone TER-119 Antibody clone TER-119, from rat, redFluor® 710

Sigma-Aldrich

MABF144A

Anti-TER-119 (mouse), redFluor® 710, clone TER-119 Antibody

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

shelf life

-

shelf life

-

density

1.119 g/mL

density

1.077 g/mL

density

-

density

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

-

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

-

application(s)

-

General description

Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL and pH 8.8–9.0.

Application

Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood.
A double gradient is formed by layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto theupper Histopaque-1077 medium. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes are found at the lowerHistopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upperplasma/Histopaque-1077 interface.It is also suitable for the purification of dendritic cells from chicken bone marrow cell suspension and neutrophils from mouse bone marrow cell suspension

Features and Benefits

Histopaque®-1119 offers numerous advantages, such as:
  • Optimal recovery of viable cells
  • Permits selective separation of blood cell types
  • Prevents cell distortion
  • Preserves cell viability
  • Minimal extraneous cell interference
  • Reproducible performance from batch to batch
  • Sterile-filtered and endotoxin-tested
  • Stable at 2-8°C for at least 2 years when protected from light

Principle

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290 - H315 - H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Ficoll®-Paque Premium Cytiva 17-5442-02, pack of 6 × 100 mL

GE17-5442-02

Ficoll®-Paque Premium

Sigma-Aldrich

Sigma-Aldrich

A1805

ACCUSPIN™ Tubes Sterile, 12 mL Capacity

ACCUSPIN™ Tubes Sterile, 50 mL Capacity

Sigma-Aldrich

A2055

ACCUSPIN™ Tubes Sterile, 50 mL Capacity

Polysucrose 400 powder

Sigma-Aldrich

P7798

Polysucrose 400

Sigma-Aldrich

Sigma-Aldrich

F4375

Ficoll® PM 400

Sigma-Aldrich

Sigma-Aldrich

P1644

Percoll®

Sigma-Aldrich

Sigma-Aldrich

A2901

Avidin–FITC from egg white

Ficoll®-Paque PREMIUM 1.073 Cytiva 17-5446-52, pack of 6 × 100 mL

GE17-5446-52

Ficoll®-Paque PREMIUM 1.073

Darin Quach et al.
Journal of bacteriology, 191(7), 2023-2032 (2008-12-31)
Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
Yuan Li et al.
BMC genomics, 16, 369-369 (2015-05-10)
Understanding genetic determinants of a microbial phenotype generally involves creating and comparing isogenic strains differing at the locus of interest, but the naturally existing genomic and phenotypic diversity of microbial populations has rarely been exploited. Here we report use of
Michael Molitor et al.
Cardiovascular research, 117(1), 162-177 (2020-02-23)
Heart failure (HF) ensuing myocardial infarction (MI) is characterized by the initiation of a systemic inflammatory response. We aimed to elucidate the impact of myelomonocytic cells and their activation by angiotensin II on vascular endothelial function in a mouse model
Ulrich Flögel et al.
Circulation, 118(2), 140-148 (2008-06-25)
In this study, we developed and validated a new approach for in vivo visualization of inflammatory processes by magnetic resonance imaging using biochemically inert nanoemulsions of perfluorocarbons (PFCs). Local inflammation was provoked in 2 separate murine models of acute cardiac
Till Stuhlmann et al.
Nature communications, 9(1), 1974-1974 (2018-05-19)
Glucose homeostasis depends critically on insulin that is secreted by pancreatic β-cells. Serum glucose, which is directly sensed by β-cells, stimulates depolarization- and Ca2+-dependent exocytosis of insulin granules. Here we show that pancreatic islets prominently express LRRC8A and LRRC8D, subunits
View All Related Papers

Protocols

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service