47036

Saponin

for molecular biology, used as non-ionic surfactant

• Synonym(s): Glycosides, Saponins
• CAS Number: 8047-15-2
• EC Number: 232-462-6
• MDL number: MFCD00081981
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• mol wt: 1000-2000 g/mol
• composition: Sapogenin, 8-25%
• technique(s): HPLC: suitable; protein quantification: suitable
• CMC: 0.001-0.01%
• density: 1.015-1.020 g/mL at 20 °C (5% in H₂O)(lit.)
• foreign activity: DNase, RNase, Protease, Phosphatase, none detected

Description

General description

Saponins are a large family of amphiphilic glycosides of steroids and triterpenes. Saponins are found in many plants and are localized especially in the root, tuber, bark, leaves, seed, and fruit. They are also present in some animals, like sea cucumber.

Application

Saponin has been used:

• in phosphate-buffered saline (PBS) to block cells for immunocytochemistry and confocal microscopy
• to permeabilize cells for measuring total mitochondrial mass
• as an internal control to extract saponin Holothuria nobilis Selenka

Biochem/physiol Actions

Saponins are nonionic surfactant that forms oil-in-water emulsions and acts as a protective colloid. They are used for the direct determination of metals in milk by flame atomic absorption spectrophotometry. Saponins are also used in permeabilization of cell membranes and separation of low molecular weight contaminants, and as an adjuvant in vaccine development.

Saponins exhibit anti-tumor, antimicrobial, and anti-herbivore activity. They may also play a key role in plant defense. Saponins also possess several pharmaceutical properties, like anti-inflammatory, antifungal, antibacterial, anti-parasitic, and antiviral activities. Saponins can regulate rhizosphere bacteria in the soil.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves