This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. Please see the link below to review further information as well as review specific protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/543/t9424bulletin.pdf
93289
TRI Reagent®
Synonym(s):
DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent
Sign In to View Organizational & Contract Pricing.
Select a Size
25 ML
1 420,00 kr
100 ML
4 310,00 kr
1 420,00 kr
Please contact Customer Service for Availability
About This Item
Skip To
form
liquid
usage
mL sufficient for 107 cells, mL sufficient for 100 mg tissue
impurities
phenol
Quality Level
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 4
This Item | T9424 | T3934 | TRIPURE-RO |
|---|---|---|---|
| grade for DNA, RNA and protein isolation | grade - | grade - | grade - |
| form liquid | form - | form - | form solution (ready-to-use) |
| usage mL sufficient for 107 cells | usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or) | usage 0.75 mL sufficient for 0.25 mL fluid samples | usage - |
| impurities phenol | impurities - | impurities - | impurities - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Application
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
Features and Benefits
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
General description
TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA.[4] This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.
Legal Information
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
related product
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Expression of adenosine receptors and vegf during angiogenesis and its inhibition by pentoxifylline?a study using zebrafish model
Nathan J, et al.
Biomedicine and Pharmacotherapy, 84(6), 1406-1418 (2016)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
Hao Zhang et al.
Molecular medicine reports, 22(2), 915-925 (2020-07-07)
Dilated cardiomyopathy (DCM) is a primary cardiomyopathy with high mortality. The aim of the present study was to identify the related genes in DCM. The four expression profiles (GSE17800, GSE21610, GSE42955 and GSE79962) downloaded from the Gene Expression Omnibus (GEO)
Ruochan Chen et al.
Science advances, 5(5), eaav5562-eaav5562 (2019-05-28)
The ability of cytosolic lipopolysaccharide (LPS) to activate caspase-11-dependent nonclassical inflammasome is intricately controlled to avoid excessive inflammatory responses. However, very little is known about the regulatory role of various metabolic pathways in the control of caspase-11 activation. Here, we
Hybrid amniotic membrane dressing with green silver nanoparticles as bioengineered skin for wounds and burns: a pilot studies
Ramesh B, et al.
Journal of biotechnology and biomaterials, 7(272), 2-2 (2017)
Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Protocols
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
-
Excuse me, what is the principle of trizol reagent to separate DNA and RNA?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service