Merck
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A2859

Sigma-Aldrich

Monoclonal Anti-ATG5 antibody produced in mouse

clone ATG5-18, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog, Anti-hAPG5
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ATG5-18, monoclonal

form

buffered aqueous solution

mol wt

antigen ~56 kDa

species reactivity

human, mouse, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)

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SAB4200508AMAB91582WH0064422M6
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

ATG5-18, monoclonal

clone

ARG1-3, monoclonal

clone

CL9508, monoclonal

clone

1F7, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

mol wt

antigen ~56 kDa

mol wt

antigen ~40 kDa

mol wt

-

mol wt

-

General description

Monoclonal Anti-ATG5 (mouse IgG2a isotype) is derived from the hybridoma ATG5-18 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Atg5, conjugated to keyhole limpet hemocyanin (KLH). The autophagy related 5 (ATG5) gene is mapped to human chromosome 6q21 and encodes a 32 kDa protein.

Specificity

Mouse monoclonal anti-ATG5 antibody reacts with human, rat, and mouse Atg5-Atg12 complex by immunoblotting (∼56kDa) and immunoprecipitation.

Application

Monoclonal Anti-ATG5 antibody produced in mouse has been used in western blotting and immunoprecipitation
Omegasomes were shown to localize to autophagosomes by immunohistochemistry using rabbit anti-ATG5 antibody.

Biochem/physiol Actions

Atg5 (also known as Apg5) is a 32kDa protein that is required for mediating autophagy. Studies have reported that the Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an important role in autophagosome formation. Furthermore, a fragment of Atg5 fragment produced by calpain cleavage is known to have pro-apoptotic properties.
Autophagy related 5 (ATG5), also known as Apg5, is essential for autophagy, a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, the autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Autophagy is required for normal turnover of cellular components during starvation. It plays an essential role in cellular differentiation, cell death and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Xian Jiang et al.
Molecular cell, 63(2), 229-239 (2016-07-23)
In response to apoptotic stimuli, mitochondria in mammalian cells release cytochrome c and other apoptogenic proteins, leading to the subsequent activation of caspases and apoptotic cell death. This process is promoted by the pro-apoptotic members of the Bcl-2 family of
Mireia Niso-Santano et al.
The EMBO journal, 34(8), 1025-1041 (2015-01-15)
To obtain mechanistic insights into the cross talk between lipolysis and autophagy, two key metabolic responses to starvation, we screened the autophagy-inducing potential of a panel of fatty acids in human cancer cells. Both saturated and unsaturated fatty acids such
Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection
Kimmey JM, et al.
Nature, 528(7583), 565-565 (2015)
Allan Sauvat et al.
EBioMedicine, 30, 261-272 (2018-04-03)
Depending on the length of their carbon backbone and their saturation status, natural fatty acids have rather distinct biological effects. Thus, longevity of model organisms is increased by extra supply of the most abundant natural cis-unsaturated fatty acid, oleic acid
Autophagy in the eukaryotic cell
Reggiori F and Klionsky DJ
Eukaryotic Cell, 1(1), 11-21 (2002)

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