A7054

ACCUSPIN^™ System-Histopaque^®-1077

• MDL number: MFCD01092091
• NACRES: NA.75

Properties

• sterility: aseptically filled
• Quality Level: 400
• form: solution
• shelf life: Expiry date on the label.
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.

Application

ACCUSPIN^™ System-Histopaque^®-1077 has been used in peripheral blood mononuclear cell isolation.

ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

Quantity

Package contains 12 prefilled tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves