Merck
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D0550

Sigma-Aldrich

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

Synonym(s):
3,5-Dinitro-2-hydroxybenzoic acid, DNS
Linear Formula:
(O2N)2C6H2-2-(OH)CO2H
CAS Number:
Molecular Weight:
228.12
Beilstein:
2220661
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

assay

≥98% (HPLC and titration)

form

powder

mp

168-172 °C (lit.)

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)

InChI key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

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Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Packaging

10, 25, 100, 500 g in glass bottle

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Flam. Sol. 1

Storage Class Code

4.1A - Other explosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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