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DNB600

Sigma-Aldrich

GenElute Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

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NACRES:
NA.52

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shipped in

ambient

storage temp.

room temp

General description

Sigma′s GenElute Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 4 mL. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

  • Isolate all sizes of circulating DNA from plasma and serum samples
  • Isolate viral and bacterial DNA
  • Versatile plasma and serum input volumes (1 mL – 4 mL)
  • Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify high-quality DNA in 40-45 minutes
  • Compatible with Streck Cell-Free DNA BCT Tubes

Application

  • PCR
  • qPCR
  • Southern Blot
  • Methylation-sensitive PCR
  • CpG array
  • Restriction enzyme digestions
  • Viral Detection
  • Bacterial Detection
  • Microarray
  • NGS

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

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