A great reagent, provides a pure pancreatic islet isolation
F9378
Ficoll® 400
Type 400-DL, lyophilized powder
Ficoll® 400
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Polysucrose 400
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5 G
823,00 kr
10 G
1 150,00 kr
25 G
2 550,00 kr
100 G
8 700,00 kr
500 G
31 000,00 kr
About This Item
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biological source
sugar beets (Beta vulgaris)
type
Type 400-DL
form
lyophilized powder
color
white
solubility
H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
storage temp.
15-25°C
Quality Level
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| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Application
Used for cell separation and organelle isolation.
Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes.[1] Ficoll 400 may be used for the development of new cell and organelle separation systems.[2] Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.[3]
Other Notes
A nonionic synthetic polymer of sucrose.
Dialyzed
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Legal Information
Ficoll is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Mirna González-González et al.
Biotechnology progress, 30(3), 700-707 (2014-01-23)
Aqueous two-phase systems (ATPS) represent a promising strategy for the recovery of CD133(+) stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon)
Yanyan Xu et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 24(4), 406-409 (2010-05-13)
The purity and activity of islets will greatly affect the outcome of xenotransplantation therapy of type 1 diabetes mellitus. To set up an improved method of the isolation and purification of rat islets, which can obtain high-purity, high-yield, and high-viability
Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Lucie Kosinova et al.
Islets, 11(6), 129-140 (2019-09-10)
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal
Frank Brand et al.
Acta neuropathologica, 139(1), 175-192 (2019-09-02)
In search of novel genes associated with glioma pathogenesis, we have previously shown frequent deletions of the KIAA1797/FOCAD gene in malignant gliomas, and a tumor suppressor function of the encoded focadhesin impacting proliferation and migration of glioma cells in vitro
Protocols
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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