H8889
Histopaque®-1077 Hybri-Max™
liquid, sterile-filtered, BioReagent, suitable for hybridoma
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UNSPSC Code:
12352201
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
Quality Level
product line
BioReagent
form
liquid
technique(s)
cell culture | hybridoma: suitable
single cell analysis: suitable
impurities
endotoxin, tested
density
1.077 g/cm3 at 25 °C (lit.)
storage temp.
2-8°C
General description
Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.
Application
Histopaque®-1077 Hybri-Max™ has been used to isolate:
- peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
- immune cells (lymphocytes) from peripheral blood
- PBMCs from blood
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
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