LUC1
Luciferase Reporter Gene Detection Kit
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1 KIT
19 340,00 kr
19 340,00 kr
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
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usage
kit sufficient for 100 assays
storage temp.
−70°C
1 of 4
This Item | SCT151 | L9420 | GUSS |
|---|---|---|---|
| usage kit sufficient for 100 assays | usage - | usage - | usage kit sufficient for 100 histochemical assays |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| storage temp. −70°C | storage temp. - | storage temp. −20°C | storage temp. −20°C |
General description
Firefly luciferase is one of the most utilized reporter genes for the study of gene expression.[1] It is an extremely sensitive, rapid, and easy-to-use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods.[2] This eliminates the need for automated luminometer injection of the substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays. The enzyme encoded by the luciferase reporter gene catalyzes the oxidation of D-luciferin in the presence of ATP, oxygen, and Mg2+. The fluorescent product is quantified to measure its activity.[2]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Current biology : CB, 21(10), 869-875 (2011-05-03)
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Stephen T Smale
Cold Spring Harbor protocols, 2010(5), pdb-pdb (2010-05-05)
When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes
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Biotechnology letters, 37(11), 2229-2235 (2015-07-15)
To improve the bioactivity and increase the N-terminal homogeneity of a glucagon-like peptide-1 (GLP-1) analogue expressed in Pichia pastoris. The GLP-1 analogue. GGH, consisting of two tandem mutant GLP-1 (GLP-1[A2G]) fused with the N-terminus of human serum albumin (HSA), was
Articles
Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
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