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N2912

Sigma-Aldrich

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

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Synonym(s):
Anti-NEF3
MDL number:
MFCD00164708
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RMdO20, monoclonal

form

buffered aqueous solution

species reactivity

mouse, rat, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extracts of rat brain S1 fraction.

isotype

IgG1

UniProt accession no.

P07197

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NEFH(4744) , NEFM(4741)
mouse ... Nefh(380684) , Nefm(18040)
rat ... Nefh(24587) , Nefm(24588)

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Anti-Neurofilament 160/200 antibody, Mouse monoclonal ~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

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N2912

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

Anti- Neurofilament 200-FITC antibody, Mouse monoclonal clone NE14, purified from hybridoma cell culture

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SAB4200811

Anti- Neurofilament 200-FITC antibody, Mouse monoclonal

Anti-Neurofilament 200 antibody, Mouse monoclonal clone NE14, purified from hybridoma cell culture

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SAB4200747

Anti-Neurofilament 200 antibody, Mouse monoclonal

Anti-Neurofilament 160 antibody, Mouse monoclonal clone NN18, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200740

Anti-Neurofilament 160 antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

-

conjugate

-

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

RMdO20, monoclonal

clone

NE14, monoclonal

clone

NE14, monoclonal

clone

NN18, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

mouse, rat, human

species reactivity

pig, feline, chicken, bovine, human, mouse, guinea pig, rat

species reactivity

feline, rat, guinea pig, pig, human, mouse, bovine, chicken

species reactivity

-

General description

Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human.

Immunogen

purified mid-size rat neurofilament (NF-M) subunit.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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