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P4937

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (25 °C), suitable for cell culture

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MDL number:
NACRES:
NA.75

sterility

aseptically filled

Quality Level

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

pH

8.5-9.5 (25 °C)

density

1.13 g/mL±0.005 g/mL at 25 °C (lit.)

storage temp.

2-8°C

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This Item
P1644P7828M6145
Sigma-Aldrich

P4937

Percoll®

Sigma-Aldrich

P1644

Percoll®

Sigma-Aldrich

P7828

Percoll®

1-Thioglycerol liquid, BioReagent, suitable for cell culture, ≥97% (titration)

M6145

1-Thioglycerol

density

1.13 g/mL±0.005 g/mL at 25 °C (lit.)

density

-

density

-

density

1.25 g/mL at 25 °C (lit.)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pH

8.5-9.5 (25 °C)

pH

8.5-9.5 (20 °C)

pH

8.9 (20 °C)

pH

-

General description

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials. Due to its heterogeneity in particle size, sedimentation occurs at different rates, spontaneously creating very smooth, isometric gradients in the range of 1.0-1.3 g/ml.

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll® has been used in the centrifugal separation of:
  • neonatal rat ventricular cardiomyocytes
  • leucocytes from a blood sample
  • peritubular myoid (PM) cells
Suitable for use in preparation of single cell suspension for sequencing.

Features and Benefits

  • Low osmolality permits precise adjustment to physiological conditions without significant interference from the medium.
  • Compatible with living cells and viruses and allows separation and recovery of intact, fully active systems.
  • Impermeable to biological membranes, resulting in no change of buoyant density of particles during centrifugation.
  • Spontaneous formation of gradient during centrifugation allows mixing of large sample volumes in the centrifuge tubes.
  • Low viscosity results in rapid formation of gradients and particle separation.
  • Most biological particles having sedimentation coefficient values greater than 60S can be successfully isolated in Percoll gradients.

Legal Information

Percoll is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
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Protocols

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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