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PROTSIL2

Sigma-Aldrich

ProteoSilver Plus Silver Stain Kit

NACRES:
NA.32

usage

1 kit sufficient for 25 mini-gels (10 × 10 cm)

Quality Level

application(s)

protein staining: suitable

storage temp.

20-25°C

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Application

ProteoSilver® Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

Features and Benefits

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Target Organs

Eyes, Respiratory system

Supplementary Hazards

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

149.0 °F - closed cup

Flash Point(C)

65 °C - closed cup

Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
Pathak P, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
Kanu Priya Aggarwal et al.
PloS one, 8(7), e69916-e69916 (2013-07-31)
The increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour....
Xiancai Ma et al.
eLife, 8 (2019-01-18)
Comprehensively elucidating the molecular mechanisms of human immunodeficiency virus type 1 (HIV-1) latency is a priority to achieve a functional cure. As current 'shock' agents failed to efficiently reactivate the latent reservoir, it is important to discover new targets for...
Peeping into human renal calcium oxalate stone matrix: characterization of novel proteins involved in the intricate mechanism of urolithiasis.
Aggarwal KP, et al.
PLoS ONE, 8, e69916-e69916 (2013)
Cédric Bernarde et al.
Molecular & cellular proteomics : MCP, 9(12), 2796-2826 (2010-07-09)
Helicobacter pylori infection plays a causal role in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma (LG-MALT) and duodenal ulcer (DU). Although many virulence factors have been associated with DU, many questions remain unanswered regarding the evolution of the...

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