Designing Safer Chemicals Learn more about the Principles of Green Chemistry.
greener alternative category
Sigma′s GenElute™ RNA/DNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Sigma′s GenElute™ RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications
Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample
Ideal for small or difficult to obtain samples
No need to split the lysate, or to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Rapid and efficient spin column procedure
Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
RT-PCR for both large mRNA and small RNA including miRNA
Expression array assays
Next Generation Sequencing
PCR-based virus detection
PCR-based viable bacteria detection
PCR and Quantitative PCR
DNA Methylation Studies
PCR-based virus detection/identification
PCR-based bacteria detection/identification
Features and Benefits
Complete column purification - The RNA, DNA and proteins are all column purified
Reduce variability - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 30 minutes
Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
Isolate high quality RNA, DNA and proteins - The purified RNA, DNA and proteins are of the highest quality and can be used in a number of downstream applications
Sustained, drug-free control of HIV-1 replication is naturally achieved in less than 0.5% of infected individuals (here termed 'elite controllers'), despite the presence of a replication-competent viral reservoir1. Inducing such an ability to spontaneously maintain undetectable plasma viraemia is a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.