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RDP300

Sigma-Aldrich

GenElute RNA/DNA/Protein Purification Plus Kit

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NACRES:
NA.52

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General description

Sigma′s GenElute RNA/DNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Sigma′s GenElute RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications

  • Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample
  • Ideal for small or difficult to obtain samples
  • No need to split the lysate, or to use phenol or precipitation methods
  • Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
  • Rapid and efficient spin column procedure

Application

RNA
  • Bioanalyzer
  • Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
  • RT-PCR for both large mRNA and small RNA including miRNA
  • Northern blotting
  • RNase protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing
  • microRNA Cloning
  • PCR-based virus detection
  • PCR-based viable bacteria detection
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR


Genomic DNA
  • PCR and Quantitative PCR
  • Sequencing
  • DNA Methylation Studies
  • Southern blotting
  • Microarrays
  • SNP analysis
  • PCR-based virus detection/identification
  • PCR-based bacteria detection/identification


Protein
  • SDS-PAGE analysis
  • Western blots
  • Mass Spectrometry

Features and Benefits

  • Complete column purification - The RNA, DNA and proteins are all column purified
  • Reduce variability - RNA, DNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
  • Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
  • Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 30 minutes
  • Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
  • Isolate high quality RNA, DNA and proteins - The purified RNA, DNA and proteins are of the highest quality and can be used in a number of downstream applications

Unit Definition

Preps

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Corrosion

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Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 1

Certificate of Analysis

Certificate of Origin

Chenyang Jiang et al.
Nature, 585(7824), 261-267 (2020-08-28)
Sustained, drug-free control of HIV-1 replication is naturally achieved in less than 0.5% of infected individuals (here termed 'elite controllers'), despite the presence of a replication-competent viral reservoir1. Inducing such an ability to spontaneously maintain undetectable plasma viraemia is a

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