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RNB300

Sigma-Aldrich

GenElute Single Cell RNA Purification Kit

greener alternative
NACRES:
NA.52

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Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

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ambient

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room temp

General description

Sigma′s GenElute Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). The kit can recover RNA from as little as a single cell to 2 x 10^5 cells. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

  • Fast and easy processing using rapid micro spin-column format
  • Small elution volume of 8 μL
  • Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
  • No phenol or chloroform extractions
  • Isolate high quality total RNA from a variety of sources
  • RNA can be isolated and detected from as little as a single animal cell

Application

  • Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
  • RT-PCR for both large mRNA and small RNA including miRNA
  • Expression profiling
  • Next Generation Sequencing for RNA and miRNA
  • microRNA cloning and amplification
  • Northern blotting
  • RNase protection
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Features and Benefits

  • Ideal for small inputs - Total RNA can be isolated from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). RNA has been isolated from as little as a single cell.
  • Smal elution volume - Elute total RNA in as little as 8 μL of Elution Buffer
  • No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without compromising yield
  • Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes

Unit Definition

Preps

Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8A - Combustible, corrosive hazardous materials

Certificate of Analysis

Certificate of Origin

Shasha Zhang et al.
Cellular and molecular life sciences : CMLS, 77(7), 1401-1419 (2019-09-06)
Foxg1 is one of the forkhead box genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. However, Foxg1 knock-out mice die at birth
Tao Gao et al.
JCI insight, 6(10) (2021-04-14)
Retinoic acid (RA) signaling is essential for enteric nervous system (ENS) development, since vitamin A deficiency or mutations in RA signaling profoundly reduce bowel colonization by ENS precursors. These RA effects could occur because of RA activity within the ENS
Dandan Liang et al.
Nature communications, 12(1), 287-287 (2021-01-14)
Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger the contraction of the heart in mammals. Though discovered over a century ago, the molecular and cellular features of the SAN that underpin its critical function in the heart are

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